Symposium and Workshop for Diagnosis-by-Sequencing using MinION 2023
Venue: International Institute for Zoonosis Control, Hokkaido university, Sapporo, JAPAN
Date: Sep 4, 2023 - Sep 7, 2023
Day 1:
10:00 Opening remarks -- Junya Yamagishi
10:10 Outline of the project -- Junya Yamagishi
10:30 Session 1 (20 min each)
Dr. Nuankanya Sathirapongsasuti, Mahidol university, Thailand
Title: Environmental DNA Sequencing by ONT for Pathogen Monitoring in Patong Beach
Dr. Orapan Sripichai, National Institute of Health, Thailand
Title: Strengthening Bacteria Infection Diagnosis, Outbreak Investigation, and Surveillance in Thailand
Dr. Ratana Tacharoenmuang, National Institute of Health, Thailand
Title: Whole genome sequencing for enhancement of Norovirus outbreak investigations in Thailand
11:30 Lunch
13:00 Session 2 (20 min each)
Dr. Mizanur Rahman, Evercare Hospital Dhaka, Bangladesh
Title: Serotype and Genetic Analysis of Dengue Virus in Severe and Non-Severe Cases in Dhaka, Bangladesh, in 2018 - 2022
Dr. Wayan T. Artama, Gadjah Mada University, Indonesia
Title: Identification of potential zoonotic pathogen from wildlife in Indonesia
Dr. Lucky Runtuwene, NIID, Japan
Title: HIV Transmitted Drug Surveillance in Japan ~Efforts to Apply NGS in HIV Research~
14:00 <break for 20 min>
14:20 Session 3 (20 min each)
Dr. Kyoko Hayashida, Hokkaido university, Japan
Title: Application of NGS for the identification of Cryptosporidium in Zambian diarrhea patients
Dr. Tatsuki Sugi, Hokkaido university, Japan
Title: 'Catch-all' test for eukaryotic pathogens in eukaryotic hosts with Nanopore
Mr. Fujihiko Minamoto, The university of Tokyo, Japan
Title: Infectious diseases in developing countries; Attempts to utilize genome technologies for better disease controls
15:20 <break for 20 min>
15:40 Session 4 (15 min each)
Ms. Tran Thi Minh Tam, National Institute of Hygiene and Epidemiology, Vietnam
Title: Nanopore application in research of acute undifferentiated febrile illnesses in Vietnam
Ms. Phan Ha My, National Institute of Hygiene and Epidemiology, Vietnam
Title: Investigation of viral etiologies in hemorrhagic fever patients
Ms. Luong Minh Hoa, National Institute of Hygiene and Epidemiology, Vietnam
Title: Diphtheria in Vietnam and Laboratory diagnosis
Mr. MD NAZMUL HASAN, Evercare Hospital Dhaka, Bangladesh
Title: Detection of Ureaplasma species in urogenital samples by PCR in suspected sexually transmitted infection patients
Mr. David Alexander, Indonesia International Institute for Life Sciences
Title: Drug-Resistant Pulmonary Tuberculosis: Current Standings in Indonesia
Ms. Dela ria nesti, Hokkaido university, Japan
Title: Development of Semi-comprehensive Method to Detect Paramyxoviruses from Indonesian Bats
Ms. MARYAM SANI LAWAL, Hokkaido university, Japan
Title: Validation of a High-throughput Metagenomic Next-Generation Sequencing Group Testing Algorithm for Comprehensive Viral Detection
17:25 Closing remarks
18:00 Welcome reception
Day 2:
Hands-on workshop for SISPA and 18S metagenomics
09:00 SISPA reaction and 18S PCR
Day 3:
Hands-on workshop for SISPA and 18S metagenomics
10:00 Library preparation for nanopore sequencer
Day 4:
Hands-on workshop for SISPA and 18S metagenomics
10:00 Data analysis
18:00 Farewell reception