Research outcomes by our lab. For a complete list of publications, please refer to the link below.↓ （external link）
Junya Yamagishi's Research map
Kyoko Hayashida's のResearch map
Tatsuki Sugi's のResearch map
2026年
⚫︎ Lawal MS, Hayashida K, Sugi T, Omoare AA, Bile N, Maikudi HI, James POI, Villeng F, Babatunde O, Idris J, Yamagishi J*.
Detection of novel Pegivirus C genome in an unexplained febrile outbreak, Gombe State, Nigeria, 2024 by enhanced mNGS approach
＞＞Int J Infect Dis. 2026 165:108470. doi: 10.1016/j.ijid.2026.108470. PMID: 41667020
◆In July 2024, an unexplaind outbreak of acute febrile illness primarily affected children in Gombe State, Nigeria.
◆Metagenomic sequencing (mNGS) of serum from 22 patients consistently detected a novel Pegivirus C variant in all samples. Genomic analysis revealed the full viral genome shares 92.7% identity with a 2018 Nigerian strain and clusters with West African isolates.
◆Although generally considered non-pathogenic, its detection in 100% of cases suggests a potential pathogenic role, warranting further investigation.
⚫︎ Chikufenji B, Hayashida K*, Goto Y, Sugi T, Sanjoba C, Njala C, McDermott I, Lohr F, Mayer D, Ogata S, Kajihara M, Nao N, Nakao R, Chimaliro L, Kapira D, Musaya J, Yamagishi J, Chatanga E.
Presence or Emergence of Canine Leishmaniasis, Malawi
＞＞Emerg Infect Dis. 2026 32(1):137-141. doi: 10.3201/eid3201.250855. PMID: 41612582
Report on the Prevalence of Canine Leishmaniasis in Malawi
◆Infection with the parasite Leishmania causes severe health consequences for both humans and dogs; however, Southern Africa has historically been considered free of this disease.
◆e recently reported the prevalence of canine leishmaniasis in Zambia (Chambaro HM and Hayashida K et al., 2025), and have now revealed that the disease is also prevalent in Malawi
◆Genetic analysis identified the causative protozoan as L. infantum zymodeme MON-1, the globally predominant genotype. This suggests it shares a common ancestor with strains confirmed in other parts of the world.
2025
⚫︎ Nesti DR, Hayashida K, Sugi T, Artama WT, Wijayanto H, Kawai N, Yamagishi J*. Development of a semicomprehensive detection method for paramyxoviruses and its validation using Indonesian bats.
＞＞ Sci Rep 15, 41249 (2025). PMID: 40450092
◆ Viral surveillance in bats is important to mitigate emerging zoonotic diseases.
◆ We developed a semicomprehensive detection method integrating group-wide RT-PCR for paramyxoviruses and multiplex next-generation sequencing.
◆ Collectively, seven sequences of novel paramyxoviruses close to Pararubulavirus, Orthorubulavirus, and Henipavirus genus were successfully identified from bat samples.
⚫︎ Sugi T, Reteng P, Gaithuma AK, Kawai N, Namangala B, Mutengo MM, Ishiguro N, Hayashida K, Yamagishi J. Enhanced blood parasite species identification using V4–V9 18S rDNA barcoding by universal primers on a nanopore platform.
＞＞ Sci Rep 15, 41249 (2025). PMID: 41271826
◆ Blood parasites, including malaria and various neglected tropical disease (NTD) parasites, remain highly prevalent, particularly in tropical regions.
◆ We combined a portable nanopore NGS device with a universal parasite 18S rDNA PCR* approach.
◆ This system enables agnostic parasite detection, including unknown or previously unrecognized parasites.
*Patent application: JP2025-005095 — Universal eukaryotic 18S rDNA amplification combined with blocking primers that selectively suppress host mammalian DNA amplification.
⚫︎ Chambaro HM¶, Hayashida K¶ (¶equally contributed), Moonga LC, Shawa M, Muleya W, Chizimu J, Squarre D, Sugi T, Yamagishi J, Ogata S, Kajihara M, Sawa H, Sanjoba C, Mwase ET, Chilengi R, Munsaka GH, Sarenje KL, Mulunda NR, Mutengo MM, Namangala B, Goto Y*. A high prevalence of dogs seropositive to Leishmania in Zambia.
＞＞ Parasitol Int. 2025. 108:103081.
A high prevalence of dogs seropositive to Leishmania in Zambia.
◆Infection with the parasite Leishmania causes severe health problems in both humans and dogs.
◆For several decades, there have been no reported cases of leishmaniasis in Zambia, Africa.
◆ Here, our recent serological survey of dogs revealed a high prevalence of Leishmania infection in the country.
List before 2023こちら