Paper Published in SciRep,Universal parasite identification method
xDevelopment of a high-throughput blood parasite screening method using a portable next-generation sequencer
⚫︎ Sugi T, Reteng P, Gaithuma AK, Kawai N, Namangala B, Mutengo MM, Ishiguro N, Hayashida K, Yamagishi J. Enhanced blood parasite species identification using V4–V9 18S rDNA barcoding by universal primers on a nanopore platform.
＞＞ Sci Rep 15, 41249 (2025). PMID: 41271826
◆ Blood parasites, including malaria and various neglected tropical disease (NTD) parasites, remain highly prevalent, particularly in tropical regions.
◆ We combined a portable nanopore NGS device with a universal parasite 18S rDNA PCR* approach.
◆ This system enables agnostic parasite detection, including unknown or previously unrecognized parasites.
*Patent application: JP2025-005095 — Universal eukaryotic 18S rDNA amplification combined with blocking primers that selectively suppress host mammalian DNA amplification.
